Miltenyi, 170-076-146, MACS® GMP Recombinant Human IL-2

Biological activity

Background information
IL-2 is a typical four α-helix bundle cytokine and is produced by activated T cells, especially the CD4+ T helper cell population. IL-2 signals through a receptor complex consisting of IL-2 receptor α-chain (CD25), β-chain, and common γ-chain.

Applications
MACS GMP Recombinant Human IL-2 can be used for a variety of applications, including the ex vivo activation and expansion of T cells, e.g., antigen-specific cytotoxic T lymphocytes2,3 or regulatory T cells4 or the ex vivo stimulation of NK cells5,6.

Technical specifications
- Identity/ Molecular mass: 15400 Da, determined by mass spectrometry. This corresponds to the mature form of human IL-2 with a cysteine-to-serine substitution at amino acid sequence position 125 (133 amino acids).
- Purity: ≥ 95 % as determined by chip electrophoresis.
- Endotoxin content: < 50 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
- Residual host cell DNA content: < 150 ng/mg, as determined by quantitative PCR specific for E. coli genomic DNA.
- Residual host cell protein content: < 0.5 µg/mg, as determined by E. coli HCP ELISA.

The product quality certificate of our MACS® GMP Recombinant Human IL-2 was adapted. Learn more here

Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer
MACS GMP Products are for ex vivo cell processing only, and are not intended for human in vivo applications. For regulatory status in the USA, please contact your local representative.

Overview
Interleukin 2 (IL-2), a potent lymphoid cell growth factor, plays an important role in both the activation and maintenance of immune responses and in lymphocyte development. IL-2 promotes, for instance, proliferation and differentiation of T cells, NK cells, and B cells.
MACS GMP Recombinant Human IL-2 is designed for ex vivo cell culture processing. No animal- or human- derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.