Description
Biological activity
Background information
SCF signals through the c-kit receptor (CD117) and exists in two forms; cell surface bound SCF and soluble SCF. The secreted soluble form of SCF is produced by the proteolytic processing of the cell surface anchored precursor molecule. SCF is constitutively produced by endothelial cells and by stromal fibroblasts.
Applications
MACS GMP Recombinant Human SCF can be used for a variety of applications, including the expansion2,3 and the viral transduction4,5 of hematopoietic stem and progenitor cells derived from bone marrow, peripheral blood cells, and cord blood, or the in vitro differentiation of megakaryocytes6.
Technical specifications
- Identity/ Molecular mass: 18.585 Da, determined by mass spectrometry. This corresponds to the mature form of human SCF including an N-terminal methionine (165 amino acids).
- Purity: ≥ 95 % as determined by chip electrophoresis.
- Endotoxin content: < 50 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
- Residual host cell DNA content: < 5 ng/mg, as determined by quantitative PCR specific for E. coli genomic DNA.
- Residual host cell protein content: < 5 µg/mg, as determined by E. coli HCP ELISA.
We have adapted the product quality certificate of our MACS® GMP Recombinant Human SCF. Learn more here
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Disclaimer
MACS GMP Products are for ex vivo cell processing only, and are not intended for human in vivo applications. For regulatory status in the USA, please contact your local representative.
Overview
Stem cell factor (SCF), also termed c-kit ligand, mast cell growth factor (MGF), or steel factor (SLF) is a hematopoietic growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and progenitor cells. Besides its pivotal role in mast cell development, SCF acts as a potent mast cell chemoattractant and upregulates mast cell adhesion and migration.
MACS GMP Recombinant Human SCF is designed for ex vivo cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.
