Description
Background information
HPVs are non-enveloped, double-stranded circular DNA viruses. Persistent infections with several HPV types are associated with the development of tumors. HPV types 16 and 18 together cause about 70 percent of cervical cancers and HPV16 infections are frequently observed in head and neck cancer patients. The viral E6 and E7 proteins of HPV16 and HPV18 are involved in the initiation and maintenance of oncogenic processes. HPV E6– and E7–specific T cell immune responses play an important role in successfully clearing HPV infection and controlling HPV-associated diseases.
Applications
The in vitro stimulation of antigen-specific T cells with PepTivator HPV16 E6 causes the secretion of effector cytokines and the up-regulation of activation markers, such as CD154 or CD137. These cytokines or activation markers then allow the detection and isolation of HPV16 E6–specific T cells.
- Detection and analysis of HPV16 E6–specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
- Isolation of viable HPV16 E6–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
- Generation of HPV16 E6–specific CD4+ and CD8+ effector/memory T cells from naive T cell populations for research on immunotherapy and vaccination.
- Pulsing of antigen-presenting cells for research on dendritic cell vaccination.
This product has not yet been tested in conjunction with the CliniMACS Cytokine Capture System (IFN-gamma).
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Disclaimer
MACS GMP Products are for ex vivo cell processing only, and are not intended for human in vivo applications. For regulatory status in the USA, please contact your local representative.
Overview
PepTivator® HPV16 E6 is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the human papillomavirus (HPV)16 E6 protein (UniProt ID: P03126).
PepTivator peptide pools have been developed for the efficient in vitro stimulation of antigen–specific CD4+ and CD8+ T cells, as peptides of 15-aa length with 11-aa overlap represent an optimized solution for stimulating both CD4+ and CD8+ T cells in various applications.
Quantitative, phenotypical, or functional analysis of HPV16 E6–specific T cell immunity can provide important information on the natural course of immune responses in healthy or immunocompromised individuals.
