Miltenyi, 170-076-161, MACS® GMP Recombinant Human IL-6

Biological activity

Background information
IL-6 is mainly produced by monocytes and macrophages, but as well by fibroblasts or endothelial cells. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 induces the terminal maturation of activated B cells into plasma cells, and in combination with other factors like IL-2 and IFN-γ it also affects cytotoxic T lymphocytes.

Applications
MACS GMP Recombinant Human IL-6 can be used for a variety of applications, including the ex vivo generation of human dendritic cells from enriched CD14+ monocytes.2–4

Technical specifications
- Identity/ Molecular mass: 20809 Da, determined by mass spectrometry. This corresponds to the mature form of human IL-6 (183 amino acids).
- Purity: ≥ 95 % as determined by chip electrophoresis.
- Endotoxin content: < 100 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
- Residual host cell DNA content: < 20 ng/mg, as determined by quantitative PCR specific for E. coli genomic DNA.
- Residual host cell protein content: < 0.5 µg/mg, as determined by E. coli HCP ELISA.

The product quality certificate of our MACS® GMP Recombinant Human IL-6 was adapted. Learn more here

Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer
MACS GMP Products are for ex vivo cell processing only, and are not intended for human in vivo applications. For regulatory status in the USA, please contact your local representative.

Overview
Interleukin 6 (IL-6) is a multifunctional cytokine, which regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.
MACS GMP Recombinant Human IL-6 is designed for ex vivo cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.