Background information
Adenoviruses (AdV) are non-enveloped, ubiquitous, lytic double‑stranded DNA viruses. Up to now, 52 different human serotypes (1–52), divided into six species (A–F), are described. AdV infections are associated with significant morbidity and mortality after hematopoetic stem cell transplantations.
The MACS GMP PepTivator AdV Select is a peptide pool of 50 lyophilized oligopeptides, consisting of MHC class I and class II restricted peptides derived from 23 proteins, of human Adenovirus (AdV) serotype -02 (E4RF3, E1B55, E1BS, E1A, DPOL, PKG3, LEAD, DNB2, PKG1, CAP6, CAPSP, CAPSH, CAP3, CAP8, Y215, COR10, SHUT, SF33K, NP, E3145, E3GL, A0A5J6SFR8) and -05 (CAPSH). The peptides are restricted for the following HLA-molecules: HLA-A*01, -A*02, -A*03, -A*24, -B*07, -B*08, -B*18, -B*35, -B*40, -B*44, -B*51, -DR1, -DR4, -DR7, -DR.
Applications
Antigen-specific T cells play a critical role in the regulation of immune responses and the elimination of virus-infected or malignant cells in the human body. Adoptive transfer of clinical-grade AdV-specific CD4+ and CD8+ T cells is a valuable tool for the treatment of AdV infections after hematopoietic stem cell transplantation (SCT).1
The in vitro stimulation of AdV-specific CD4+ and CD8+ T cells with MACS GMP PepTivator AdV Select causes the production of the effector cytokine IFN-gamma.
For the enrichment of AdV-specific effector/memory T cells with the use of the CliniMACS Cytokine Capture System (IFN-gamma), we recommend to use the MACS GMP PepTivator AdV hexon (170-076-106).
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Disclaimer
MACS GMP Products are for ex vivo cell processing only, and are not intended for human in vivo applications. For regulatory status in the USA, please contact your local representative.
Overview
The MACS GMP PepTivator AdV Select is a peptide pool that consists of 50 lyophilized oligopeptides, consisting of MHC class I and class II restricted peptides derived from different proteins of human adenovirus (AdV), serotypes 2 and 5. It has been developed for efficient in vitro stimulation and subsequent isolation of AdV-specific CD4+ and CD8+ T cells.
