Description
Biological activity
Background information
Sonic hedgehog (SHH) is involved in a wide variety of embryonic developmental events, including neurogenesis, limb development, hematopoiesis, and gut formation. Besides their role as morphogens, hedgehog family members have also been shown to act as mitogens, cell survival factors, and axon guidance factors and to influence tissue regeneration within the adult. SHH is synthesized as a precursor, which is autocatalytically cleaved into a 19 kDa N-terminal domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N is responsible for the signaling activity of hedgehog proteins, while SHH-C acts as a cholesterol transferase and covalently attaches a cholesterol molecule to SHH-N. The lipid-modified SHH-N displays enhanced activity. Human SHH (C24II) corresponds to the mature SHH-N domain and carries a Cys to Ile-Ile substitution resulting in a lipophilic moiety and therefore an increased activity.
Applications
MACS GMP Recombinant Human SHH (C24II) can be used for a variety of applications including:
Expansion or differentiation of various cell types, mainly human and mouse embryonic stem cells, but also adult stem cells and mesenchymal cells. Human SHH (C24II) is an essential growth factor for the differentiation of dompaninergic progenitors from PSCs.
Technical specifications
- Identity/ Molecular mass: 19.683 Da, determined by mass spectrometry. This corresponds to the mature form of human SHH (C24II) (175 amino acids).
- Purity: ≥ 95 % as determined by chip electrophoresis.
- Product related impurities: ≤ 5 % as determined by chip electrophoresis.
- Endotoxin content: < 50 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
- Residual host cell DNA content: < 50 ng/mg, as determined by quantitative PCR specific for E. coli genomic DNA.
- Residual host cell protein content: < 0.5 µg/mg, as determined by E. coli HCP ELISA.
The product quality certificate of MACS® GMP Recombinant Human SHH (C24II) was adapted. Learn more here
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Disclaimer
MACS GMP Products are for ex vivo cell processing only, and are not intended for human in vivo applications. For regulatory status in the USA, please contact your local representative.
Overview
Recombinant Human SHH (Sonic Hedgehog; or C24II) can promote growth and differentiation of embryonic stem cells, adult mesenchymal stem cells, and cells within the neuronal lineage. The morphogenetic cytokine is crucial for embryonic development, as it is involved in the patterning of the central nervous system, somites, and limbs.
MACS GMP Recombinant Human SHH (C24II) is designed for ex vivo cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective product quality certificate. The product is lyophilized without carrier protein or preservatives.
