Description
Biological activity
Background information
EGF is the prototype of the large family of EGF-like proteins with a common structural motif comprising three intramolecular disulfide bonds. EGF is produced by various cell types like mammary gland cells, gut epithelial cells, and cells in the nervous system and the kidney. Production of EGF is induced by testosterone and inhibited by estrogens. In vitro, EGF stimulates the proliferation and differentiation of mesenchymal cells, acts as a mitogen for fibroblasts, neural precursors, epithelial and endothelial cells, and promotes colony formation of epidermal cells.
Applications
MACS GMP Recombinant Human EGF can be used as an ingredient for many cell culture applications. EGF can support the maintenance of ectodermal and mesodermal progenitor cells, and stimulates the proliferation and differentiation of a wide variety of cell types deriving both from ectoderm and mesoderm.
Technical specifications
- Identity/ Molecular mass: 6347 Da, determined by mass spectrometry. This corresponds to the mature form of human EGF including an N-terminal methionine (54 amino acids).
- Purity: ≥ 95 % as determined by HPLC-SEC.
- Endotoxin content: < 50 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
- Residual host cell DNA content: < 20 ng/mg, as determined by quantitative PCR specific for E. coli genomic DNA.
- Residual host cell protein content: < 10 µg/mg, as determined by E. coli HCP ELISA.
The product quality certificate of MACS® GMP Recombinant Human EGF was adapted. Learn more here
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Disclaimer
MACS GMP Products are for ex vivo cell processing only, and are not intended for human in vivo applications. For regulatory status in the USA, please contact your local representative.
Overview
Epidermal growth factor (EGF) is a small mitogenic polypeptide that stimulates the proliferation and differentiation of a wide variety of cells of ectodermal and mesodermal origin, including fibroblasts, epithelial cells, and endothelial cells.
MACS GMP Recombinant Human EGF is designed for ex vivo cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.
