Description
Background information
Cells sorted on the MACSQuant Tyto Cell Sorter remain inside the closed MACS® GMP Tyto® Cartridge HS at all times during the sort process. The cartridge consists of three compartments that can each be accessed through individual, aseptic Luer-Locks from above: the input chamber, the positive collection chamber, and the negative collection chamber. Cells suspended in specially formulated MACS GMP Tyto Running Buffer are loaded into the input chamber, which contains an adjustable propeller to keep cells in suspension during the sort.
After loading the cartridge into the instrument, filtered air is applied to the input chamber through a pressure inlet. Driven by comparatively low air pressure (17‒18 psi), fluorescently labeled cells flow from the input chamber through the microchip via an inertial focusing channel. At the microchip, cells are interrogated by three lasers. Fluorescent and scatter light signatures are used to select target cells, which are then redirected by a sort valve located within the microchannel.
To redirect a target cell into the positive collection chamber, a magnetic field is applied to the microchip. This triggers the sorting valve to open and therefore changes the flow of the target cell towards the positive collection chamber. After a target cell has passed through, a silicon spring returns the valve to its original position.
Unlike during conventional droplet sorting procedures, cells sorted on the MACSQuant Tyto Cell Sorter are not subjected to high pressures, are not decompressed, and have no electrical charge applied, resulting in superior viability and functionality of cells.
The MACS GMP Tyto Cartridge HS is specifically designed to facilitate never-before-seen high-speed cell sorting protocols on the MACSQuant Tyto Cell Sorter, shortening the required sample processing time significantly. In addition, due to its cutting-edge microfluidic pathway, the MACS GMP Tyto Cartridge HS also features inertial cell focusing to improve sorting performance.
As in the standard MACS GMP Tyto Cartridge, the patented microchip technology in the MACS GMP Tyto Cartridge HS enables fluorescence-based and aerosol-free cell sorting with the world’s fastest mechanical sort valve (up to 30,000 valve actuations per second) in a fully closed and sterile environment, providing complete operator safety.
The MACS GMP Tyto Cartridge HS is a single-use disposable that diminishes any risk of sample cross-contamination. Both positive and negative sorted fractions remain in the MACS GMP Tyto Cartridge HS after the actual sort, allowing full retrieval of all cells within the sample.
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Disclaimer
MACS GMP Products are for ex vivo cell processing only, and are not intended for human in vivo applications. For regulatory status in the USA, please contact your local representative.
Overview
The sort process on the MACSQuant® Tyto® Cell Sorter happens exclusively within a closed and disposable cartridge system. The MACS GMP Tyto Cartridge HS was specifically developed for high-speed, GMP-compliant cell sorting, and therefore simplifies transfer of protocols between research and GMP settings. This allows for total flexibility in the implementation and translation of newly developed applications.
ATTENTION: To be able to run the MACS GMP Tyto Cartridge HS on your MACSQuant® Tyto® Cell Sorter, MACSQuantify™ Tyto Software version 0.5.1 or higher is required! For further information, please contact Technical Support
