Proteintech, 10021-1-Ig-150ul, 5 Lipoxygenase Polyclonal antibody

10021-1-Ig targets 5 Lipoxygenase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: DU 145 cells, HL-60 cells, K-562 cells, PC-3 cells, human placenta tissue
Positive IHC detected in: human lung cancer tissue, human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: HepG2 cells
Recommended dilution
Western Blot (WB): WB : 1:500-1:1000
Immunohistochemistry (IHC): IHC : 1:200-1:800
Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
Background Information
ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).