Proteintech, 10622-1-AP-20ul, 14-3-3 Sigma Polyclonal antibody

10622-1-AP targets 14-3-3 Sigma in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications
Positive WB detected in: A431 cells, A549 cells, HeLa cells, U2OS cells, MCF-7 cells
Positive IHC detected in: mouse skin tissue, human bowen diseaseNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Recommended dilution
Western Blot (WB): WB : 1:20000-1:100000
Immunohistochemistry (IHC): IHC : 1:250-1:1000
Background Information
14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.