Proteintech, 11604-1-AP-150ul, ALDH4A1 Polyclonal antibody

11604-1-AP targets ALDH4A1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: human heart tissue, HepG2 cells, human brain tissue, human liver tissue, human skeletal muscle tissue, K-562 cells, mouse skeletal muscle tissue, mouse small intestine tissue, rat heart tissue
Positive IP detected in: mouse skeletal muscle tissue
Positive IHC detected in: human liver cancer tissue, human liver tissue, human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Recommended dilution
Western Blot (WB): WB : 1:500-1:2400
Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC): IHC : 1:50-1:500
Background Information
ALDH4A1(Aldehyde dehydrogenase family 4 member A1) is also named as ALDH4, P5CDH,P5C dehydrogenase and belongs to the aldehyde dehydrogenase family. P5CDH is a mitochondrial matrix NAD(+)-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate.Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2).