Proteintech, 12406-1-AP-150ul, Aconitase 1 Polyclonal antibody

12406-1-AP targets Aconitase 1 in WB, IHC, IF-P, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: HeLa cells, mouse liver tissue, rat liver tissue
Positive IP detected in: HepG2 cells
Positive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: mouse liver tissue
Recommended dilution
Western Blot (WB): WB : 1:1000-1:4000
Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC): IHC : 1:200-1:800
Immunofluorescence (IF)-P: IF-P : 1:50-1:500
Background Information
Mammalian cells possess two distinct isoforms of aconitase encoded by different genes: Aconitase 1 [ACO1; cytosolic aconitase (c-aconitase)] encoded by ACO1 and Aconitase 2 [ACO2; mitochondrial aconitase (m-aconitase)] encoded by ACO2. In addition to the enzymatic roles in the cytoplasm, ACO1 also is known to bind to mRNAs of specific genes and maintain iron homeostasis.