Proteintech, 12965-1-AP-20ul, ACP6 Polyclonal antibody

12965-1-AP targets ACP6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications
Positive WB detected in: A2780 cells, mouse liver tissue, COLO 320 cells, HepG2 cells, RT-4 cells, SKOV-3 cells
Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Recommended dilution
Western Blot (WB): WB : 1:500-1:3000
Immunohistochemistry (IHC): IHC : 1:20-1:200
Background Information
Lysophosphatidic acid phosphatase type 6 (ACP6) is an LPA-specific acid phosphatase that hydrolyzes LPA to monoacylglycerol (MAG) and phosphate. Previous studies have shown that ACP6 is localized in the mitochondria with ubiquitous expression throughout all tissues, and high expression levels in kidney, heart, small intestine, muscle, and liver. Through specifi cally hydrolyzing LPA, ACP6 regulates mitochondrial lipid biosynthesis, thereby controlling important mitochondrial functions. It was also shown that ACP6 might be involved in tumor progression, suggesting that ACP6 could be a good candidate marker for the prognosis of esophageal cancer. Uniprot showed that by alternative splicing, ACP6 may have two kinds of shear fragments with molecular weights of 49 and 31kDa respectively. (PMID: 23807634)