Proteintech, 13328-1-AP-150ul, ADA Polyclonal antibody

13328-1-AP targets ADA in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications
Positive WB detected in: A431 cells, HeLa cells, Jurkat cells, Sp2/0 cells
Positive IHC detected in: human stomach tissue, human cervical cancer tissue, human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: mouse small intestine tissue
Recommended dilution
Western Blot (WB): WB : 1:1000-1:4000
Immunohistochemistry (IHC): IHC : 1:20-1:200
Immunofluorescence (IF)-P: IF-P : 1:50-1:500
Background Information
Adenosine deaminase (ADA, EC 3.5.4.4), which has been found in plants, bacteria, and mammals including humans and others, catalyzes the hydrolysis of adenosine to yield inosine. It was demonstrated that ADA also catalyzes the hydrolysis of the derivatives of nucleoside adenosine such as 2′-deoxyadenosine. ADA is expressed on the surface of T and B cells and plays an important role in the development of immune system. (PMID: 26038697)