Proteintech, 13815-1-AP-20ul, AACS Polyclonal antibody

13815-1-AP targets AACS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: mouse heart tissue, mouse liver tissue
Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: MCF-7 cells
Recommended dilution
Western Blot (WB): WB : 1:200-1:1000
Immunohistochemistry (IHC): IHC : 1:50-1:500
Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
Background Information
AACS(acetoacetyl-CoA synthetase) is also named as ACSF1 and belonogs to the ATP-dependent AMP-binding enzyme family. In mammals, AACS is present in the cytosolic fractions of various tissues, especially lipogenic ones, and acetoacetate is known to be effectively incorporated into cholesterol and fatty acids. These results indicate that AACS may supply acetyl units to cytosolic compartment for cholesterol and/or fatty acid biosynthesis. It has 3 isoforms produced by alternative splicing.