Proteintech, 13880-1-AP-20ul, Aggrecan Polyclonal antibody

13880-1-AP targets Aggrecan in IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive IHC detected in: human lung tissue, human cartilage tissue, human lung cancer tissue, mouse lung tissue, rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive FC (Intra) detected in: ATDC-5 cells
Recommended dilution
Immunohistochemistry (IHC): IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Background Information
Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.