Proteintech, 14181-1-AP-150ul, Alpha 1B-Glycoprotein/A1BG Polyclonal antibody

14181-1-AP targets Alpha 1B-Glycoprotein/A1BG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications
Positive WB detected in: human plasma tissue, BxPC-3 cells, MCF-7 cells, mouse kidney tissue
Positive IHC detected in: human hepatocirrhosis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: HepG2 cells
Recommended dilution
Western Blot (WB): WB : 1:2000-1:20000
Immunohistochemistry (IHC): IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
Background Information
Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).