Proteintech, 14395-1-AP-20ul, Alpha smooth muscle actin Polyclonal antibody

14395-1-AP targets Alpha smooth muscle actin in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA, competitive binding assay applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: mouse colon tissue, human placenta tissue, A549 cells, HUVEC cells, rat colon tissue, mouse heart tissue, rat heart tissue, mouse liver tissue, mouse kidney tissue
Positive IP detected in: mouse liver tissue
Positive IHC detected in: human appendicitis tissue, human colon tissue, human heart tissue, human hysteromyoma tissue, human liver cancer tissue, mouse colon tissue, stromal tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: mouse heart tissue, mouse small intestine tissue
Positive IF-Fro detected in: mouse colon tissue
Positive IF/ICC detected in: C2C12 cells, H9C2 cells
Positive FC (Intra) detected in: C2C12 cells
Recommended dilution
Western Blot (WB): WB : 1:2000-1:12000
Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC): IHC : 1:4000-1:16000
Immunofluorescence (IF)-P: IF-P : 1:1000-1:4000
Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICC: IF/ICC : 1:800-1:3200
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Background Information
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.