Proteintech, 14674-1-AP-150ul, ADHFE1 Polyclonal antibody

14674-1-AP targets ADHFE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: HT-29 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue
Positive IHC detected in: human colon tissue, human colon cancer tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Recommended dilution
Western Blot (WB): WB : 1:500-1:2000
Immunohistochemistry (IHC): IHC : 1:100-1:400
Background Information
The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues. It belongs to the iron-containing alcohol dehydrogenase family. ADHFE1 has 4 isoforms produced by alternative splicing with the MW of 50, 45, 32, 27 kDa.