Proteintech, 14725-1-AP-20ul, AHSA1 Polyclonal antibody

14725-1-AP targets AHSA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: A431 cells, HeLa cells, mouse brain tissue, HEK-293 cells, A549 cells, rat brain tissue
Positive IP detected in: mouse brain tissue
Positive IHC detected in: mouse skeletal muscle tissue, human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: A431 cells
Recommended dilution
Western Blot (WB): WB : 1:500-1:3000
Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC): IHC : 1:100-1:500
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800