Proteintech, 15618-1-AP-150ul, AKAP1 Polyclonal antibody

15618-1-AP targets AKAP1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: Caco-2 cells, human liver tissue, HEK-293 cells, HeLa cells, MCF-7 cells, PC-3 cells, SW480 cells
Positive IP detected in: PC-3 cells
Positive IHC detected in: human lung cancer tissue, human prostate cancer tissue, human colon cancer tissue, human small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: MCF-7 cells
Recommended dilution
Western Blot (WB): WB : 1:1000-1:4000
Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC): IHC : 1:50-1:500
Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
Background Information
AKAP1 (also termed AKAP149) is a human 149 kDa anchoring protein localized in mitochondria and the endoplasmic reticulum/nuclear envelope (ER-NE) network. It Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.