Description
16046-1-AP targets ALG5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: human kidney tissue, mouse liver tissue
Positive IHC detected in: mouse liver tissue, human liver cancer tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: HEK-293 cells
Recommended dilution
Western Blot (WB): WB : 1:500-1:1000
Immunohistochemistry (IHC): IHC : 1:50-1:500
Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
Background Information
Dolichyl-phosphate beta-glucosyltransferase(ALG5), belongs to the family of glycosyltransferases, specifically the hexosyltransferases. ALG5 is widely expressed in pancreas, placenta, liver, heart, brain, kidney, skeletal muscle, and lung. ALG5 can catalyze the chemical reaction between UDP-glucose and UDP with dolichyl phosphate.