Proteintech, 16087-1-AP-20ul, ACSS2 Polyclonal antibody

16087-1-AP targets ACSS2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: HepG2 cells, HEK-293 cells, mouse brain tissue, mouse liver tissue, rat liver tissue, Neuro-2a cells, rat brain tissue, L02 cells, U-87 MG cells
Positive IP detected in: HepG2 cells
Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC): IHC : 1:50-1:500
Background Information
Acetyl-CoA Synthetase 2 (ACSS2) is also named as acyl-CoA synthetase short-chain family member 2. Acetyl-CoA Synthetase 2 (ACSS2) is responsible for lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis and promotes acetylation in nuclear (PMID: 29444517). It is also required for cell migration and invasion in renal cell carcinoma (RCC) through the promotion of autophagy (PMID: 29281714). ACSS2 has two isoforms with the molecular weight of 78 and 80 kDa.