Proteintech, 17521-1-AP-20ul, ACTA1-Specific Polyclonal antibody

17521-1-AP targets ACTA1-Specific in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected in: human skeletal muscle tissue, human hysteromyoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive FC (Intra) detected in: C2C12 cells
Recommended dilution
Western Blot (WB): WB : 1:500-1:2000
Immunohistochemistry (IHC): IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Background Information
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.