Proteintech, 18302-1-AP-20ul, Angiogenin Polyclonal antibody

18302-1-AP targets Angiogenin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications
Positive WB detected in: mouse liver tissue, human milk tissue
Positive IHC detected in: human liver tissue, human Hepatocellular carcinomaNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Recommended dilution
Western Blot (WB): WB : 1:200-1:1000
Immunohistochemistry (IHC): IHC : 1:50-1:500
Background Information
Angiogenin (ANG), an angiogenic ribonuclease, is a member of the vertebrate-specific, secreted RNASE superfamily. Angiogenin, originally identified as a tumor angiogenic factor, was related with the growth and metastasis of numerous tumors. Angiogenin has been proposed as a permissive factor for angiogenesis induced by other angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor, acidic fibroblast growth factor, and epidermal growth factor. Angiogenin production and secretion may be stimulated by hypoxia. Increased angiogenin serum levels have been associated with the incidence and severity of several human tumors, including HCC. It is a 17 kDa precursor which is cleaved to generate the 14 kDa mature protein.