Proteintech, 18897-1-AP-150ul, ADH1C Polyclonal antibody

18897-1-AP targets ADH1C in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: rat liver tissue
Positive IP detected in: mouse liver tissue
Positive IHC detected in: rat liver tissue, human lung cancer tissue, human colon tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: HepG2 cells
Recommended dilution
Western Blot (WB): WB : 1:2000-1:12000
Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC): IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
Background Information
ADH1C(Alcohol dehydrogenase 1C) is also named as ADH3 and belongs to the zinc-containing alcohol dehydrogenase family.It catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde.The ADH1C gene is active in intestine and kidney in fetal and early postnatal life, and persists in the stomach and liver in adult life(PMID:4748759).