Proteintech, 19492-1-AP-20ul, ADCY3 Polyclonal antibody

19492-1-AP targets ADCY3 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
Tested Applications
Positive WB detected in: mouse kidney tissue
Positive IP detected in: MDCK cells
Positive IHC detected in: human kidney tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: rat brain tissue
Positive IF/ICC detected in: hTERT-RPE1 cells, MDCK cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC): IHC : 1:20-1:200
Immunofluorescence (IF)-P: IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Background Information
ADCY3 is an adenylate cyclase (AC) functioning to convert ATP to cAMP in response to signals initiated by activation of Gs-coupled receptors. To date, 10 isoforms of AC have been cloned in mammals. Initially identified in olfactory cilia, ADCY3 is regarded as the olfactory isoform and required for detection of odorants. Recently it has been found that outside of the olfactory epithelium the olfactory-like signaling pathway including AC3 may also have a key role in spermatogenesis and spermatozoa functions. In addition, the ciliary location of AC3 makes it as a prominent marker for primary cilia in brain.AC3 is typically found at 130 kDa in olfactory tissues.In nonolfactory tissues (such as sperm), this protein is reported to migrate with a molecular mass of 55 kDa,occasionally accompanied by additional bands at either 90 or 130 kDa(PMID:9539154).