Proteintech, 22147-1-AP-150ul, ADAM5 Polyclonal antibody

22147-1-AP targets ADAM5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: HeLa cells, MCF-7 cells
Positive IHC detected in: human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: HeLa cells
Recommended dilution
Western Blot (WB): WB : 1:500-1:2000
Immunohistochemistry (IHC): IHC : 1:20-1:200
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Background Information
ADAM5(putative disintegrin and metalloproteinase domain-containing protein 5) is also named as ADAM5P, TMDC2(tMDCII). The tMDC II, abundantly expressed, sperm surface MDC proteins identified in rodent and macaque systems, is also nonfunctional in the human(PMID:10417343). It has 7 isoforms produced by alternative splicing. It is expressed in testis highly by northern blot. This antibody is specific to ADAM5.