Proteintech, 22401-1-AP-20ul, ACSL4/FACL4 Polyclonal antibody

22401-1-AP targets ACSL4/FACL4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: COLO 320 cells, BV-2 cells, HEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, RAW 264.7 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected in: HEK-293 cells
Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC): IHC : 1:50-1:500
Background Information
Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).