Proteintech, 22670-1-AP-150ul, A4GNT Polyclonal antibody

22670-1-AP targets A4GNT in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: K-562 cells, mouse pancreas tissue, mouse stomach tissue, rat stomach tissue, L02 cells
Positive IHC detected in: human stomach cancer tissue, human pancreas cancer tissue, rat stomach tissue, mouse stomach tissue, human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Recommended dilution
Western Blot (WB): WB : 1:500-1:1000
Immunohistochemistry (IHC): IHC : 1:50-1:500
Background Information
A4GNT (alpha-1,4-N-acetylglucosaminyltransferase) catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans, forming a unique glycan structure, GlcNAcalpha1-->4Galbeta-->R. The gene is expressed with a bias in certain tissues, particularly in the stomach (RPKM 9.5) and duodenum (RPKM 0.9). Alpha4GnT has been implicated in O-glycan biosynthesis and is thought to have a role in preventing gastric cancer by inhibiting Helicobacter pylori infection and suppressing tumor-promoting inflammation.