Proteintech, 23168-1-AP-20ul, AFAF/EQTN Polyclonal antibody

23168-1-AP targets AFAF/EQTN in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: HepG2 cells, HeLa cells
Positive IF/ICC detected in: HepG2 cells
Recommended dilution
Western Blot (WB): WB : 1:500-1:2000
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Background Information
The AFAF gene (also known as EQTN and C9orf11) encodes a membrane protein that is specifically localized to the acrosomal membrane. AFAF is involved in the process of fertilization and acrosome biogenesis. Expression analysis demonstrates that AFAF is highly expressed in the testis, although minor expression was seen in other tissues. AFAF has 3 isoforms with molecular weights of 14, 29, and 33 kDa. However, one article detected molecular weight between 40-65 kDa in mouse testis (PMID: 19605790).