Proteintech, 23660-1-AP-20ul, Alpha Actin Polyclonal antibody

23660-1-AP targets Alpha Actin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected in: mouse skeletal muscle tissue
Positive IHC detected in: mouse skeletal muscle tissue, human colon tissue, human heart tissue, human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: mouse heart tissue, mouse skeletal muscle tissue
Positive IF/ICC detected in: AC16 cells, H9C2 cells
Positive FC (Intra) detected in: C2C12 cells
Recommended dilution
Western Blot (WB): WB : 1:20000-1:100000
Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC): IHC : 1:200-1:800
Immunofluorescence (IF)-P: IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension