Proteintech, 23724-1-AP-150ul, AIDA Polyclonal antibody

23724-1-AP targets AIDA in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications
Positive WB detected in: HEK-293 cells, HeLa cells
Positive IP detected in: HeLa cells
Positive IHC detected in: mouse skeletal muscle tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Recommended dilution
Western Blot (WB): WB : 1:500-1:1000
Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC): IHC : 1:50-1:500
Background Information
AIDA, also named as C1orf80, acts as a ventralizing factor during embryogenesis. It inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. It's highest expression in the heart and skeletal muscle. AIDA has three isoforms with MW 35 kDa, 16 kDa and 26 kDa.