Proteintech, 23778-1-AP-20ul, ADAM8 Polyclonal antibody

23778-1-AP targets ADAM8 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications
Positive WB detected in: BxPC-3 cells, mouse pancreas tissue
Positive IHC detected in: human tonsillitis tissue, human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Recommended dilution
Western Blot (WB): WB : 1:500-1:1000
Immunohistochemistry (IHC): IHC : 1:20-1:200
Background Information
ADAMs are a large family of transmembrane proteins that are involved in proteolysis, making them candidates for mediating the remodeling of the extracellular matrix(ECM). ADAM8, one of the members of the ADAM family, is overexpressed in various human tumors. It has been shown previously that hypoxia influences the expression of some ADAM proteins. The protein found to be expressed in two active forms(90 kDa and 60 kDa): potential shedding protein and remnant protein in the pancreatic tissues under chronic pancreatitis and pancreatic cancer(PMID:18566576). The full length protein has a signal peptide and four glycosylation sites. This antibody is specific to ADAM8.