Proteintech, 24333-1-AP-20ul, ANKRD6 Polyclonal antibody

24333-1-AP targets ANKRD6 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: HEK-293 cells, fetal human brain tissue
Positive IP detected in: HEK-293 cells
Positive IHC detected in: mouse ovary tissue, human kidney tissue, human ovary tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Recommended dilution
Western Blot (WB): WB : 1:200-1:1000
Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC): IHC : 1:50-1:500
Background Information
Ankrd6 (Ankyrin repeat domain 6) is a distant homolog of the fly PCP mediator diego and acts as a molecular switch between both Wnt pathways. It promotes signaling of the non canonical Wnt/PCP pathway and simultaneously inhibits gene transcription through the canonical Wnt/β-catenin pathway.