Proteintech, 24550-1-AP-150ul, AMOT Polyclonal antibody

24550-1-AP targets AMOT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: HEK-293 cells, HEK-293T
Positive IP detected in: HEK-293 cells
Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: HEK-293 cells
Recommended dilution
Western Blot (WB): WB : 1:1000-1:9000
Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC): IHC : 1:50-1:500
Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
Background Information
Angiomotin belongs to the motion family of angiostatin-binding proteins. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells during the formation of new blood vessels. The most abundant expression was found in the placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). The p130 isoform can interact with F-actin. This antibody recognizes both p130 and p80.