Proteintech, 25167-1-AP-150ul, ALDH1A3 Polyclonal antibody

25167-1-AP targets ALDH1A3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: HepG2 cells, mouse liver tissue, rat liver tissue
Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: A549 cells
Recommended dilution
Western Blot (WB): WB : 1:500-1:2000
Immunohistochemistry (IHC): IHC : 1:50-1:500
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Background Information
Aldehyde dehydrogenase 1 family member A3 (ALDH1A3), is an aldehyde dehydrogenase enzyme that uses retinal as a substrate either in a free or a cellular retinol-binding protein form. ALDH1A3 plays a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation.ALDH1A3 is calculated to be 56 kDa.