Proteintech, 25414-1-AP-20ul, AFF2 Polyclonal antibody

25414-1-AP targets AFF2 in WB, IP, ELISA applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: SH-SY5Y cells
Positive IP detected in: SH-SY5Y cells
Recommended dilution
Western Blot (WB): WB : 1:500-1:2000
Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Background Information
AFF2 (OMIM* 300806) (also known as FMR2 gene), which encodes AF4/FMR2 family member 2, is a transcriptional factor and RNA-binding protein that plays an important role in transcriptional regulation, RNA splicing, mRNA processing, and nuclear speckle organization . AFF2 is highly conserved and abundantly expressed in human brain, being essential for brain development. Homozygous AFF2 knock-out mice showed abnormal central nervous system synaptic transmission, abnormal excitatory postsynaptic potential, and premature death (PMID: 35431806).