Description
25566-1-AP targets ALKBH8 in IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
Tested Applications
Positive IHC detected in: rat kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: U2OS cells, HEK-293 cells
Recommended dilution
Immunohistochemistry (IHC): IHC : 1:50-1:500
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Background Information
ALKBH8 (Alpha-ketoglutarate-dependent dioxygenase) is unique among the ALKBH family, since it harbors a 2-oxoglutarate and Fe(II)-dependent oxygenase domain as well as an RNA recognition motif and methyl transferase domain (PMID: 27329810). ALKBH8 is a "writer" enzyme that modifies the wobble uridine of tRNA and regulates protein translation, including that of selenoproteins (PMID: 38550277). ALKBH8 is highly expressed in high-grade, superficially and deeply invasive bladder cancer. ALKBH8 knockdown induces apoptosis in bladder cancer cells (PMID: 38550277).