Proteintech, 31080-1-AP-20ul, ANK2 Polyclonal antibody

31080-1-AP targets ANK2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: HEK-293 cells, mouse heart tissue, rat heart tissue
Positive IHC detected in: mouse heart tissue, mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: mouse heart tissue
Recommended dilution
Western Blot (WB): WB : 1:2000-1:16000
Immunohistochemistry (IHC): IHC : 1:50-1:500
Immunofluorescence (IF)-P: IF-P : 1:50-1:500
Background Information
The Ankyrins, belonging to the adapter protein family, consist of Ankyrin-R (ANK1), Ankyrin-B (ANK2), and Ankyrin-G (ANK3). Initially, ANK2 was considered as a key cytoskeletal-associated protein in the brain. Besides, it was discovered as a pivotal adaptor and scaffolding protein in the heart. Remarkably, recent studies have demonstrated that Ankyrins contribute to the modulation of various biological activities in cancers. (PMID: 36539782)