Proteintech, 33283-1-AP-20ul, Ace Polyclonal antibody

33283-1-AP targets Ace in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.
Tested Applications
Positive WB detected in: mouse lung tissue
Positive IHC detected in: mouse lung tissue, rat lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: rat lung tissue, mouse lung tissue
Recommended dilution
Western Blot (WB): WB : 1:2000-1:16000
Immunohistochemistry (IHC): IHC : 1:500-1:2000
Immunofluorescence (IF)-P: IF-P : 1:50-1:500
Background Information
ACE(Angiotensin-converting enzyme) is also named as DCP, DCP1 and belongs to the peptidase M2 family. It is involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. ACE also has a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. It has 4 isoforms produced by alternative splicing. The full length protein has 15 glycosylation sites and it can show a molecular weight of 170 kDa (PMID:16804085).