Proteintech, 55135-1-AP-150ul, Alpha smooth muscle actin specific Polyclonal antibody

55135-1-AP targets Alpha smooth muscle actin specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: mouse colon tissue, mouse kidney tissue, mouse stomach, rat colon, mouse lung, rat lung
Positive IHC detected in: mouse heart tissue, human colon tissue, human colon cancer tissue, human heart tissue, human hepatocirrhosis tissue, human liver cancer tissue, mouse colon tissue, mouse liver tissue, rat colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: mouse heart tissue
Recommended dilution
Western Blot (WB): WB : 1:500-1:4000
Immunohistochemistry (IHC): IHC : 1:500-1:3000
Immunofluorescence (IF)-P: IF-P : 1:50-1:500
Background Information
ACTA2, also named as a-SMA,AAT6;ACTSA belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6). The immunogen of this antibody is a specific domain of ACTA2. This antibody stains the aorta only.