Proteintech, 55268-1-AP-20ul, Alpha Parvin/Actopaxin Polyclonal antibody

55268-1-AP targets Alpha Parvin/Actopaxin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
Tested Applications
Positive WB detected in: U2OS cells, HeLa cells, mouse kidney tissue
Positive IP detected in: U2OS cells
Positive IHC detected in: human stomach cancer tissue, human heart tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: MDCK cells
Recommended dilution
Western Blot (WB): WB : 1:200-1:1000
Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC): IHC : 1:50-1:500
Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
Background Information
α-Parvin (PARVA), also named as MXRA2, Actopaxin and CH-ILKBP, belongs to the parvin family. It probably plays a role in the regulation of cell adhesion and cytoskeleton organization. (PMID:11134073) This antibody is specific to α-Parvin.