Proteintech, 60240-1-Ig-150ul, AMACR/p504S Monoclonal antibody

60240-1-Ig targets AMACR/p504S in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: HepG2 cells, PC-3 cells, HEK-293 cells, HeLa cells, LNCaP cells, HSC-T6 cells, 4T1 cells, NIH/3T3 cells
Positive IF/ICC detected in: A431 cells
Recommended dilution
Western Blot (WB): WB : 1:1000-1:6000
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Background Information
AMACR(Alpha-methyl acyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing. Defects in AMACR are the cause of alpha-methyl acyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).