Proteintech, 65181-1-Ig-500ug, Anti-Human CD146/MCAM (P1H12)

65181-1-Ig targets CD146/MCAM in WB, IF/ICC, FC applications and shows reactivity with human, mouse, rabbit, canine samples.
Tested Applications
Positive IF/ICC detected in: HUVEC cells
Positive FC detected in: HeLa cells
Recommended dilution
Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension
Background Information
CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).