Proteintech, 66125-1-Ig-20ul, Alpha cardiac muscle actin specific Monoclonal antibody

66125-1-Ig targets Alpha cardiac muscle actin specific in WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications
Positive WB detected in: human heart tissue, pig heart tissue, rat heart tissue, mouse heart tissue
Positive IHC detected in: human heart tissue, human heart and human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: mouse heart tissue
Positive IF-Fro detected in: mouse heart tissue
Positive FC (Intra) detected in: C2C12 cells
Recommended dilution
Western Blot (WB): WB : 1:2500-1:10000
Immunohistochemistry (IHC): IHC : 1:100-1:400
Immunofluorescence (IF)-P: IF-P : 1:200-1:800
Immunofluorescence (IF)-FRO: IF-FRO : 1:400-1:1600
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Background Information
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.