Proteintech, 66126-1-Ig-20ul, Alpha-2-Macroglobulin Monoclonal antibody

66126-1-Ig targets Alpha-2-Macroglobulin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: HepG2 cells, human brain tissue
Positive IP detected in: human plasma tissue
Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: HepG2 cells
Recommended dilution
Western Blot (WB): WB : 1:500-1:2000
Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC): IHC : 1:1000-1:32000
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Background Information
alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.