Proteintech, 66200-1-Ig-150ul, Acetyl-Tubulin (Lys40) Monoclonal antibody

66200-1-Ig targets Acetyl-Tubulin (Lys40) in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, canine samples.
Tested Applications
Positive WB detected in: rat testis tissue, NCCIT cells, Neuro-2a cells, L02 cells, pig cerebellum tissue, rat brain tissue, mouse ovary tissue, HEK-293 cells, mouse testis
Positive IHC detected in: mouse ovary tissue, human gliomas tissue, rat brain tissue, mouse brain tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF-P detected in: islet, mouse brain tissue
Positive IF/ICC detected in: MDCK cells, hTERT-RPE1 cells
Positive ELISA detected in: Recombinant protein
Recommended dilution
Western Blot (WB): WB : 1:2000-1:10000
Immunohistochemistry (IHC): IHC : 1:1000-1:4000
Immunofluorescence (IF)-P: IF-P : 1:10-1:100
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:10-1:100
Background Information
What is the molecular weight of acetyl-α-tubulin?The molecular weight of acetylated tubulin is 52 kD.Where does the acetylation of Lys 40 occur?This acetylation occurs inside the microtubule lumen by the α-tubulin acetyltransferase 1 (αTAT1) (PMID: 29207274).How can acetylation be reversed?Acetylation of Lys 40 can be reversed by deacetylase 6 (HDAC6), which is mostly cytoplasmic that also deacetylates Hsp90, and sirtuin 2 (SIRT2), which also mainly cytoplasmic and uses NAD as a coenzyme. Unlike HDAC6, SIRT uses both polymerized and soluble tubulin as substrates. Deacetylases are believed to be more active on soluble tubulin, while acetylases function preferentially on stable polymers (PMIDs: 29207274, 30079247, 19185337).What is the function of acetylation?Acetylation is a conserved post-translational modification of alpha tubulin at Lys 40 during tubulin assembly, and it correlates to increased microtubule stability and intracellular transport (PMIDs: 29207274, 30079247, 20940043).Is acetylation of α-tubulin strictly associated with stable microtubules?Not necessarily, as acetylation can have other effects on microtubule subpopulations (PMID 20940043).Is ac-tubulin found only in cilia?Acetylated-α-tubulin is located in cytoplasmic tubulin as well as in cilia; therefore, it is not strictly region-specific (PMID: 30079247).What are the cellular effects of tubulin acetylation?Microtubule acetylation seems to provide a critical role in neuronal development and function, and while its effect on cancer cells remains unclear, it has been shown that decreased acetylated α-tubulin impairs neuronal cell line migration. The post-translational modification may also help regulate organelle-independent signalling throughout the cell, supporting the notion of a microtubule network serving as a coordinator of cellular signaling (PMIDs: 29207274, 25503560, 20940043, 19185337).