Proteintech, 66251-1-Ig-20ul, 14-3-3 Sigma Monoclonal antibody

66251-1-Ig targets 14-3-3 Sigma in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications
Positive WB detected in: A-253 cells, A431 cells, HeLa cells, MCF-7 cells, HT-1376 cells, HaCaT cells, SCaBER cells, MCF-10A cells, MDA-MB-468 cells
Positive IHC detected in: human skin tissue, human breast cancer tissue, human breast hyperplasia tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: MCF-7 cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunohistochemistry (IHC): IHC : 1:200-1:8000
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Background Information
14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.