Proteintech, 66532-1-Ig-20ul, ACOT9 Monoclonal antibody

66532-1-Ig targets ACOT9 in WB, IHC, ELISA applications and shows reactivity with Human, Rat, mouse samples.
Tested Applications
Positive WB detected in: HEK-293 cells, RAW 264.7 cells, Jurkat cells, HSC-T6 cells
Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Recommended dilution
Western Blot (WB): WB : 1:5000-1:20000
Immunohistochemistry (IHC): IHC : 1:50-1:500
Background Information
ACOT9 (Acyl-coenzyme A thioesterase 9) is a member of the acyl-CoA thioesterase superfamily, which is a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. ACOT9 has 4 isoforms with the molecular mass of 43-50 kDa.